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Influence of Sodium‐Independent γ‐Aminobutyric Acid Binding on the Assay of Sodium‐Dependent γ‐Aminobutyric Acid Binding in a Membrane Preparation of Rat Brain
Author(s) -
Kurioka Susumu,
Matsuda Makoto
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb02413.x
Subject(s) - sodium , aminobutyric acid , chemistry , binding site , membrane , biochemistry , gamma aminobutyric acid , receptor , biophysics , biology , organic chemistry
A large amount of [ 3 H]GABA was bound to crude synaptic membrane fractions of rat. by sodium‐independent process in a medium that contained 100 μM [ 3 H]GABA used for assaying GABA uptake site. This [ 3 H]‐GABA binding was different from receptor binding of GABA. It was confirmed that this sodium‐independent [ 2 H]GABA binding scarcely occurred in the presence of a physiological concentration of sodium chloride, and that sodium‐independent GABA binding had a negligible influence on sodium‐dependent GABA binding.