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Studies on Lipase in Rat Brain
Author(s) -
Mizobuchi Masato,
Shirai Kohji,
Matsuoka Nobuo,
Saito Yasushi,
Kumagai Akira
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb02409.x
Subject(s) - lipase , neuroscience , biology , chemistry , biochemistry , enzyme
Lipase activity was measured in homogenates of rat cerebral hemispheres using radioactive glycerol trioleate emulsified with Triton X‐100 as substrate. The labeled oleic acid was separated from the ester with a methanol‐chloroform‐heptane mixture. Under these assay conditions, the activity showed pH optima at about 5.5 and 7.5. The final products of these lipase activities were suggested to be free fatty acid and glycerol.