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Characterization of Alkylgalactolipids from Calf Brain by High Performance Liquid Chromatography
Author(s) -
Yahara Shoji,
Kishimoto Yasuo
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb02394.x
Subject(s) - chromatography , chemistry , alkyl , thin layer chromatography , column chromatography , diglyceride , high performance liquid chromatography , fatty acid , countercurrent chromatography , paper chromatography , cerebroside , organic chemistry , biochemistry
Minor nonpolar galactolipids were isolated from the total lipids of calf brain stem by column chromatography and were separated by preparative thin‐layer chromatography into four groups. The material recovered from the bottom band of the thin‐layer chromatography consisted of monogalactosyl diglyceride and its 1‐0‐alkyl isomer, alkylgalactolipid, present in a molar ratio of 11 :9. After perbenzoylation. they were separated by preparative thin‐layer chromatography and characterized. The fatty acid compositions of these lipids were similar to each other and to those of the ester‐linked fatty acids of cerebroside esters. The major alkyl group of alkylgalactolipid was palmityl, and the other, minor components were oleyl. myristyl, and stearyl ethers. Perbenzoylated derivatives of these lipids were further separated by reverse‐phase high performance liquid chromatography. The chromatograms from these two lipids were similar; however, most of the peaks were still mixtures of homologs containing different fatty acids or an alkyl group.