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Ibotenic Acid Analogues as Inhibitors of [ 3 H]Glutamic Acid Binding to Cerebellar Membranes
Author(s) -
Honoré Tage,
Lauridsen Jørn,
KrogsgaardLarsen Povl
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb01735.x
Subject(s) - ibotenic acid , glutamic acid , chemistry , potency , membrane , in vivo , binding site , biochemistry , stereochemistry , amino acid , in vitro , biology , central nervous system , endocrinology , microbiology and biotechnology
The L‐[ 3 H]glutamic acid binding capability of rat cerebellar membranes prepared with or without preincubation at 37°C followed by washing was investigated. The two preparations ( K D = 820 nM, B max = 54.5 pmol/mg protein; K D = 509 nM, B max =13.0 pmol/mg protein) showed no difference in specificity of the binding of the ibotenic acid analogues, consistent with the removal of an endogenous inhibitor by the preincubation at 37°C followed by washing. The order of potency of the ibotenic acid analogues as inhibitors of L‐[ 3 H]glutamic acid binding is different from the order of potency in vivo , suggesting that the binding sites found are different from the physiological glutamic acid receptor.