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Norepinephrine Metabolism in Man Using Deuterium Labelling: The Conversion of 4‐Hydroxy‐3‐Methoxyphenylglycol to 4‐Hydroxy‐3‐Methoxymandelic Acid
Author(s) -
Mårdh Göran,
Sjöquist Birgitta,
ÄnggÅrd Erik
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb01716.x
Subject(s) - chemistry , deuterium , labelling , chromatography , biochemistry , quantum mechanics , physics
4‐Hydroxy‐3‐methoxyphenylglycol (HMPG) labelled with three deuterium atoms was used to study the disposition of peripherally administered HMPG. Five healthy men were given an intravenous pulse dose of 4.3 μmol of labelled HMPG and subsequent plasma and urine levels of endogenous and labelled HMPG as well as those of 4‐hydroxy‐3‐methoxymandelic acid (HMMA, VMA) were determined by gas chromatography‐mass spectrometry, using selected ion detection. Approximately 40% of the injected amount of deuterium‐labelled HMPG was recovered in the urine as HMMA and another 40% was eliminated as HMPG conjugates. Thus, the HMPG formed from norepinephrine either in the central or peripheral nervous system undergoes both conjugation and extensive oxidation.