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Ontogeny of Histaminergic Neurotransmission in the Rat Brain: Concomitant Development of Neuronal Histamine, H‐1 Receptors, and H‐1 Receptor‐Mediated Stimulation of Phospholipid Turnover
Author(s) -
Subramanian N.,
Whitmore W. L.,
Seidler F. J.,
Slotkin T. A.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb01710.x
Subject(s) - histaminergic , histamine , histamine h3 receptor , histamine receptor , biology , histamine h1 receptor , endocrinology , medicine , receptor , histamine h2 receptor , stimulation , neurotransmission , thioperamide , agonist , biochemistry , antagonist
The ontogeny of histaminergic neurotransmission in the rat brain was studied by assessing development of histamine levels in brain regions, along with H‐1 receptor binding of [ 3 H]mepyramine and H‐1 receptor‐mediated cellular events. In the hypothalamus, which is rich in histaminergic innervation, levels of the amine were low at birth, increased sharply at 8 days of age, and reached adult concentrations shortly thereafter; this pattern is typical of most neurotransmitters. In contrast, regions poor in neuronal histamine showed an initially high histamine level and a subsequent decline with development, as is known to occur during general growth of tissues. The developmental profile of H‐1 receptor binding sites resembled that of the neuronal histamine pool, and the increases with age resulted from changes in the number of binding sites without alterations in K d . Cellular responses to H‐1 receptor activation were assessed by determining the stimulation of phospholipid turnover evoked by intracisternally administered histamine, a process that has been shown to involve only the neuronal compartment. Again, the developmental profile was superimposable upon that of H‐1 receptor binding and that of hypothalamic histamine levels. These studies indicate that ontogeny of histaminergic neurotransmission is a coordinated process, with simultaneous development of neuronal histamine, its key biosynthetic enzyme, and H‐1 receptors coupled directly to cellular events.