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Studies on γ‐Aminobutyric Acid Transport in Cobalt Experimental Epilepsy in the Rat
Author(s) -
Ross Stephen M.,
Craig Charles R.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb01693.x
Subject(s) - cobalt , in vivo , chemistry , epilepsy , gamma aminobutyric acid , in vitro , biophysics , biochemistry , medicine , endocrinology , pharmacology , receptor , biology , neuroscience , inorganic chemistry , microbiology and biotechnology
Crude brain homogenates and cerebral tissue slices from rats with cobalt metal implanted in right and left cerebral cortices were used to examine high‐ and low‐affinity GABA transport. High‐affinity GABA transport was maximally reduced to 34% of controls 7 days after cobalt implantation, a time that coincides with peak seizure activity in this model. Kinetic analysis of high‐affinity GABA transport, using brain homogenates, revealed a significant change in V max 7 days after cobalt implantation. (V max = 446.4 ± 26.2 pmol/mg prot./min, cobalt, versus 787.8 ± 67.3, control). An analysis of the low‐affinity system revealed no depression of K m , or V max parameters. Administration of valproic acid at a concentration as high as 1 mM in vitro or a dose of 300 mg/kg in vivo had no effect on high‐ or low‐affinity GABA transport. The results obtained from cobalt‐treated rats provide additional evidence for an involvement of GABA in experimental epilepsy.