Premium
Characterization and Purification from Human Brain of a Hyaluronic Acid‐Binding Glycoprotein, Hyaluronectin
Author(s) -
Delpech Bertrand,
Halavent Catherine
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb01672.x
Subject(s) - hyaluronic acid , glycoprotein , biochemistry , chemistry , human brain , microbiology and biotechnology , neuroscience , biology , anatomy
Using affinity chromatography and enzyme‐labelled immunological assays combined with aftinity adsorption, we have obtained evidence for the binding of a brain glycoprotein to hyaluronic acid, and on this basis named it hyaluronectin. This binding was inhibited by hyaluronic acid and by the products of its hydrolysis by hyaluronidase from bovine testis, but was not inhibited by other glycosaminoglycans or by monosaccharides. Preparative affinity chromatography of brain acid‐soluble proteins produced hyaluronectin in a good degree of purity. Contamination by albumin was less than 1% and the yield was as high as 80%.