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Effects of Selective Alkylphosphorylation of Propionylcholinesterase on the Regeneration of Acetylcholinesterase in the Aqueous Soluble Fraction of Superior Cervical Ganglia of the Rat Following Sarin
Author(s) -
Koelle Winifred Angenent,
Rickard Kathleen Kitto,
Koelle George B.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb01607.x
Subject(s) - sarin , acetylcholinesterase , aché , chemistry , cervical ganglia , regeneration (biology) , pharmacology , endocrinology , medicine , biochemistry , biology , enzyme , microbiology and biotechnology
The rates of regeneration of acetylcholinesterase (AChE) and propionylcholinesterase (PrChE) in the supernatants of aqueous homogenates of rat superior cervical ganglia, centrifuged at 100,000 g for 90 min, were determined at 1,3, 6, and 16 h following their inactivation (>90%) by administration of sarin, 2.0μmol/kg i.v. Values were compared with those in animals in which the PrChE was continually suppressed by the repeated, fractional administration of iso‐OMPA, in a total dose of 10 or 20 μmol/kg i.p. These doses of iso‐OMPA alone produced 96–99% inactivation of PrChE with no detectable effect on AChE. Significant suppression of AChE regeneration by iso‐OMPA administration was noted only at 6 h; in contrast with earlier findings in the cat, administration of iso‐OMPA alone caused no significant increase in ganglionic AChE activity.