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Myo ‐Inositol Turnover in the Intact Rat Brain: Increased Production after d ‐Amphetamine
Author(s) -
Barkai Amiram I
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00590.x
Subject(s) - inositol , amphetamine , endocrinology , medicine , endogeny , perfusion , chemistry , in vivo , metabolism , biology , receptor , dopamine , microbiology and biotechnology
Apparent turnover of myo ‐inositol in the brain of urethane‐anesthetized rats was estimated in vivo from the rate of appearance of endogenous myo ‐inositol in the cerebroventricular compartment. Ventricular‐cisternal perfusion technique combined with isotope dilution of [ 14 C] myo ‐inositol was used to determine the rate of appearance of brain‐produced myo ‐inositol and its modification by d ‐amphetamine. A mean value of 0.75 nmol/min was obtained for the rate of appearance in the cerebroventricular system. A dose dependent increase in this rate was seen after the administration of d ‐ampheta‐mine. The endogenous removal of myo ‐inositol from the perfusate was also studied and found to be mediated in part by a saturable transport system that was not influenced by d‐amphetamine. The rate of entry of myo ‐inositol from blood to the erebroventricular system was very low and accounted for only 2% of the total rate of appearance, indicating that the majority of myo ‐inositol in the rat cerebroventricular fluid originates in the brain.