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A Comparison of the Polypeptide Pattern Between a Brain Primary Culture and Fractions of Bulk‐Isolated Glial Cells
Author(s) -
Hansson Elisabeth,
Rönnbäck Lars,
Sellström Åke
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00588.x
Subject(s) - primary culture , biology , cell culture , biochemistry , electrophoresis , neuroglia , microbiology and biotechnology , central nervous system , endocrinology , in vitro , genetics
This paper reports on the electrophoretic protein/polypeptide pattern of a rat brain primary culture. For comparison, the polypeptide pattern of neuronal and glial enriched fractions from adult rat brain and cerebral hemispheres from newborn and adult rat have been analysed. Water‐soluble and SDS‐extractable polypeptide fractions appeared and/or increased in amount in the cultures until confluency. The polypeptide pattern of the cultures most resembled that of the glial cell fractions, showing some of this fraction's specificity. Removal of fetal calf serum and addition of 0.1 mM dibutyryl cyclic adenosine monophosphate (dB‐cAMP) produced few changes in the electrophoretic pattern. The study thus provides evidence in favour of the astroglial nature of the brain primary culture. It also shows that the cells undergo some maturation in the culture.