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Regional and Subcellular Localization in Rat Brain of the Enzymes That Can Synthesize γ‐Hydroxybutyric Acid
Author(s) -
Rumigny J. F,
Maitre M,
Cash C,
Mandel P
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00583.x
Subject(s) - enzyme , subcellular localization , neuroscience , biochemistry , chemistry , biology , microbiology and biotechnology , cytoplasm
Rat brain contains two major NADPH‐linked aldehyde reductases that can reduce succinate semialdehyde to 4‐hydroxybutyrate. One of these enzymes appears to be fairly specific for succinate semialdehyde and is not significantly inhibited by classic aldehyde reductase inhibitors such as barbiturates. The other enzyme can reduce several aromatic aldehydes and is strongly inhibited by barbiturates and branched‐chain fatty acids. Using one such inhibitor, it was possible to distinguish between and measure the two enzyme activities separately in various rat brain regions and in subcellular fractions. Both enzymes are mainly cytoplasmic but there is some activity in the synaptosomal fraction. The activity of the specific succinic semialdehyde reductase is highest in the cerebellum, where it represents 21% of the total activity, and lowest in the cortex, where it represents about 11% of the total activity.