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Turnover of Myelin Proteins of Rat Brain, Determined in Fractions Separated by Sedimentation in a Continuous Sucrose Gradient
Author(s) -
Shapira R,
Wilhelmi M. R,
Kibler R. F
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00582.x
Subject(s) - myelin , chemistry , sucrose , membrane , biochemistry , density gradient , dilution , leucine , chromatography , polyacrylamide gel electrophoresis , specific activity , sodium , sucrose gradient , biology , central nervous system , endocrinology , amino acid , enzyme , physics , thermodynamics , organic chemistry , quantum mechanics
Rats that Received intracranial injections of [ 3 H]leucine at 14 days of age were killed on days 17, 24, 38, 55, and 89 post‐injection. Brains were homogenized and the myelin membranes separated in a sucrose density gradient. At day 17 sodium dodecylsulfate polyacrylamide gels of water‐shocked, delipidated membrane fractions showed a difference in the specific activity of myelin proteins across the gradient. A decrease in specific activity was found in all of the proteins in the denser fractions, compared with the lighter fractions. As time after injection progressed, the difference became more pronounced; a two‐ to threefold decrease in specific activity was seen across the gradient in the various myelin proteins. The proteins of the lightest membrane fractions retained their high specific activity throughout the experiment in spite of extensive new myelin synthesis. Taking this new myelin into account, the decrease in specific activity in the denser myelin fractions could be explained by isotope dilution. Therefore, proteins present in at least some of the myelin are essentially stable.