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A Rapid and Simple Method for the Determination of Picogram Levels of 3‐Methoxytyramine in Brain Tissue Using Liquid Chromatography with Electrochemical Detection
Author(s) -
Ponzio F,
Achilli G,
Algeri S
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00573.x
Subject(s) - chemistry , chromatography , nomifensine , extraction (chemistry) , high performance liquid chromatography , monoamine oxidase , biochemistry , enzyme , dopamine , endocrinology , biology , dopaminergic
A rapid and simple technique using solvent extraction, ion‐pairing extraction, and high pressure liquid chromatography with electrochemical detection has been developed for the determination of 3‐methoxytyramine in striata of rats killed by microwave irradiation. The method is specific and reproducible (coefficient of variation among replications, ±4%); recovery of authentic 3‐methoxytyramine added to the samples is 45–50%. 3‐Methoxytyramine levels found with this technique in rat striata were 15 ± 1.7 ng/g. The method has a sensitivity of about 0.2 pmol per brain sample. Monoamine oxidase inhibition with pargyline increased 3‐methoxytyramine levels in rat striata, while catechol‐ O ‐methyltransferase inhibition with 3′,4′‐dihydroxy‐2 methylpropiophenone completely depleted 3‐methoxytyramine. The effects of nomifensine, quipazine, caroxazone, piribedil, and D‐amphetamine were also examined. The 3‐methoxytyramine concentrations in the brains of animals killed by decapitation or by microwave irradiation were compared.