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Brain Tubulin Microheterogeneity in the Mouse During Development and Aging
Author(s) -
Hungen Kern,
Chin Rustum C.,
Baxter Claude F.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00485.x
Subject(s) - tubulin , neuroscience , biology , microtubule , chemistry , microbiology and biotechnology
Mouse brain tubulin was analyzed on isoelectric focusing gels. High‐resolution gels utilizing Bio‐Rad ampholytes (pH 4‐6) revealed 5‐6 bands in the region corresponding to the α‐subunit of tubulin and 10 or more for the β‐subunit. The same general banding pattern was observed regardless of the method of preparation of the tubulin. Two species prominent in the brains of immature mice, α6 and β2, virtually disappeared during maturation, while species β6 to β10 appeared. No significant changes from the mature pattern were seen during aging (examined at 12, 23, and 30 months of age).