Premium
Enzymic Lipid Peroxidation in the Microsomal Fraction of Rat Brain
Author(s) -
Player T. J.,
Horton A. A.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00472.x
Subject(s) - lipid peroxidation , microsome , biochemistry , microsoma , chemistry , hydroxylation , catalase , cytochrome , reductase , superoxide dismutase , enzyme
An enzymic lipid peroxidation system has been demonstrated in the microsomal fraction of rat brain and the requirements and optimal conditions for assay determined. The involvement of NADPH‐cytochrome c reductase was demonstrated in vesicles reconstituted with lipids extracted from the brain microsomal fraction. Further characterization of the system made use of substances shown to inhibit the liver microsomal system. α‐Tocopherol was shown to be an effective inhibitor of lipid peroxidation in the brain microsomal system, whereas Na 2 SO 3 had no effect, which is indicative that free radical transfer occurs only in the hydrophobic regions. Neither superoxide dismutase nor catalase inhibited lipid peroxidation. The implications of an NADPH‐cytochrome c reductase‐dependent lipid peroxidation system that is not linked to a drug hydroxylation system and appears to differ from the liver microsomal system in a number of other ways are discussed.