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Identification of a Mitochondrial Phosphoprotein in Brain Synaptic Membrane Preparations
Author(s) -
Magilen Gilbert,
Gordon Adrienne,
Au Alice,
Diamond Ivan
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00442.x
Subject(s) - phosphoprotein , synaptic membrane , identification (biology) , mitochondrion , neuroscience , neurotransmission , chemistry , biology , biochemistry , membrane , phosphorylation , receptor , botany
A 41,000‐dalton phosphoprotein in crude synaptosomal membrane fractions is characterized by its unique divalent and monovalent cation regulation. It is identified by two‐dimensional gel electrophoresis as the phosphoprotein whose phosphorylation is enhanced by repetitive electrical stimulation of hippocampal brain slices. After sucrose‐gradient ultracentrifugation, this phosphoprotein is found in the mitochondrial subfraction. This suggests that the electrically produced changes in the level of phosphorylation of the 41,000‐dalton polypeptide are probably effects on cellular energetics rather than on specialized neural membrane function.