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[ 3 H]Choline Uptake and Metabolism in Nonsynaptic Regions of a Crustacean Sensory Nerve
Author(s) -
Auerbach Anthony,
Barker David L.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00415.x
Subject(s) - choline , acetylcholine , cholinergic , biophysics , protonophore , intracellular , phosphorylcholine , chemistry , biology , biochemistry , neuroscience , endocrinology , membrane potential
The posterior stomach nerve (PSN) is a crustacean sensory nerve containing about 60 cholinergic neurons, which are devoid of synaptic interactions. Kinetic analysis shows that the PSN takes up [ 3 H]choline by both low‐affinity ( K m = 163 μM) and high‐affinity (Na + ‐dependent) ( K m = 1 μM) processes. The capacity of the high‐affinity system is only about 1% that of the low‐affinity system. The high‐affinity system is not tightly coupled to acetylcholine (ACh) synthesis, and it appears that both ACh and phosphorylcholine are formed from an intracellular pool of choline, which is fed by both uptake systems. There are differences in the rates of [ 3 H]choline uptake and 3 H metabolite accumulation between regions of the PSN that contain neuronal cell bodies and those that do not. These differences may arise from differences in the relative proportion of neuronal to nonneuronal tissue in each nerve region.