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High‐Affinity Uptake of Spermine by Slices of Rat Cerebral Cortex
Author(s) -
Harman R. J.,
Shaw G. G.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb00409.x
Subject(s) - spermine , ouabain , sodium cyanide , biophysics , potassium cyanide , dinitrophenol , cerebral cortex , sodium , polyamine , chemistry , cyanide , central nervous system , biochemistry , biology , neuroscience , enzyme , inorganic chemistry , organic chemistry
The accumulation of the polyamine spermine into 0.1‐mm prisms of rat cerebral cortex has been investigated at both 37°C and at 4°C. Kinetic analysis of the temperature‐sensitive portion of uptake indicates two high‐aftinity saturable components together with an unsaturable component at high concentrations. The ‘very high’– affinity saturable system ( K m = 3.8 nM) was temperature‐ and sodium‐dependent, and significantly reduced by metabolic inhibitors, findings that are consistent with an active transport system for spermine into brain tissue. The ‘high’– affinity saturable component ( K m = 0.44 μM) was sodium‐dependent and inhibited by ouabain, but only partially susceptible to inhibition by 2,4‐dinitrophenol and sodium cyanide. The significance of these results with respect to the function of spermine in the central nervous system is discussed.