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The Use of Sep‐Pak™ C 18 Cartridges During the Isolation of Gangliosides
Author(s) -
Williams Marcia A.,
McCluer Robert H.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb12515.x
Subject(s) - cartridge , chloroform , chromatography , chemistry , methanol , aqueous solution , elution , adsorption , aqueous two phase system , salt (chemistry) , organic chemistry , materials science , metallurgy
The use of Sep‐Pak™ C 18 reverse‐phase cartridges to adsorb gangliosides from aqueous solutions was studied. When upper phases formed from chloroform‐methanol tissue extracts or aqueous salt solutions containing gangliosides are rapidly passed through the cartridges, the lipids are retained and the non‐lipid components can be washed through. Gangliosides and other retained lipids can subsequently be eluted with methanol or chloroform‐methanol.