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Acetyl‐CoA Synthesizing Enzymes in Cholinergic Nerve Terminals
Author(s) -
Sterri Sigrun H.,
Fonnum Frode
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb12511.x
Subject(s) - fumarase , choline acetyltransferase , atp citrate lyase , pyruvate dehydrogenase complex , biochemistry , citrate synthase , pyruvate dehydrogenase phosphatase , pyruvate dehydrogenase kinase , transaminase , acetyltransferase , enzyme , cholinergic , biology , lactate dehydrogenase , citric acid cycle , acetyl coa , chemistry , endocrinology , gene , acetylation
The activities of five enzymes involved in acetyl‐CoA synthesis, pyruvate dehydrogenase complex, ATP citrate lyase, carnitine acetyl‐transferase, acetyl‐CoA synthetase, and citrate synthase, were determined in normal nucleus interpeduncularis and nucleus interpeduncularis in which cholinergic terminals were removed following lesion of the habenulo‐interpeduncular tract. The activities of aspartate transaminase, fumarase, and GABA transaminase also were determined to compare the effect of lesion on other mitochondrial enzymes which are not linked to the biosynthesis of ACh. In normal nucleus interpeduncularis the activities of carnitine acetyltransferase and pyruvate dehydrogenase complex were higher than the activity of ChAT (choline acetyltransferase), whereas the activities of acetyl‐CoA synthetase and citrate synthase were considerably lower than that of ChAT. The effect of the lesion separated the enzymes into two groups: the activities of pyruvate dehydrogenase complex, carnitine acetyltransferase, fumarase and aspartate transaminase decreased by 30–409%, whereas the activities of the other enzymes decreased 5–15%. ChAT activity was in all cases less than 159% of normal. It could be concluded that none of the acetyl‐CoA synthesizing enzymes decreased to the degree that ChAT did. Only pyruvate dehydrogenase complex and carnitine acetyltransferase seem to be localized in cholinergic terminals to a significant degree. ATP citrate lyase as well as acetyl‐CoA synthetase seem to have less significance in supporting acetyl‐CoA formation in cholinergic nerve terminals.