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Effects of Detergents, Proteins, and Lipids on 2′:3′‐Cyclic‐Nucleotide 3′‐Phosphodiesterase Activity
Author(s) -
Clapshaw Patric A.,
Seifert Wilfried
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb12502.x
Subject(s) - phosphodiesterase , enzyme , cyclic nucleotide , biochemistry , cyclic nucleotide phosphodiesterase , nucleotide , myelin , chemistry , myelin basic protein , stimulation , enzyme assay , biology , endocrinology , central nervous system , gene
The myelin marker 2′:3′‐cyclic‐nucleotide 3′‐phosphodiesterase (CNPase) was isolated to a lipid‐ and phosphate‐free stage. The effects of exogenously added lipids were tested on this preparation and compared to the known stimulation of the enzyme by detergents and proteins. CNPase could be stimulated 2–3 fold by these various agents which appeared to be additive in their effect. Enzyme‐protein and enzyme‐lipid interactions and possible medical use of the improved assay conditions for CNPase employed in the study are discussed.