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Hydrogen Peroxide Production by Rat Brain In Vivo
Author(s) -
Sinet Pierre M.,
Heikkila Richard E.,
Cohen Gerald
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb11222.x
Subject(s) - catalase , pargyline , in vivo , monoamine oxidase , chemistry , hydrogen peroxide , biochemistry , reserpine , enzyme , biology , endocrinology , microbiology and biotechnology
H 2 O 2 production by rat brain in vivo was observed with a method based on the measurement of brain catalase. The administration to the rat of 3‐amino‐1, 2, 4‐triazole, an H 2 O 2‐ dependent inhibitor of catalase, caused progressive inhibition of brain catalase activity in both the supernatant and pellet fractions of homogenates of the striatum and prefrontal cortex. The prevention of catalase inhibition by prior administration of ethanol confirmed that catalase inhibition in vivo was dependent upon H 2 O 2 . A significant portion of the catalase (30‐33%) appeared in the supernatant fraction from a slow‐speed homogenization procedure and was not significantly contaminated by either erythrocytes or capillaries. In the whole homogenate, less than 6% of the catalase activity was attributed to erythrocytes. Modification of intracellular monoamine oxidase activity by either pargyline or reserpine did not change the rate of inhibition of catalase by aminotriazole. A probable interpretation of these data is that H 2 O 2 generated by mitochondrial monoamine oxidase does not reach the catalase compartment; the catalase is contained in particles described by other investigators as the microperoxisomes of brain. In studies in vitro , the production of H 2 O 2 by rat brain mitochondria with either dopamine or serotonin as substrate was confirmed.