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Measurement of the Membrane Potential of Isolated Nerve Terminals by the Lipophilic Cation [ 3 H]Triphenylmethylphosphonium Bromide
Author(s) -
Hansson Elisabeth,
Jacobson Ingemar,
Venema Richard,
Sellström Åke
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb11182.x
Subject(s) - chemistry , bromide , membrane potential , ouabain , membrane , ionophore , incubation , biophysics , inhibitory postsynaptic potential , chromatography , biochemistry , inorganic chemistry , sodium , biology , organic chemistry , neuroscience
The lipophilic cation [ 3 H]triphenylrnethylphosphonium bromide ([ 3 H]TPMP + ) was investigated as a measure of the membrane potential of synaptosomes. Conditions under which [ 3 H]TPMP + achieved an equilibrium distribution were tested. The toxicity of TPMP has been studied relative to its inhibitory effects on [ 3 H]y‐aminobutyric acid ([ 3 H]GABA) transport. In some experiments the distribution of 86 RbZ + and [ 3 H]TPMP + was changed upon incubation in the presence of elevated levels of K + , ouabain, or KCN, or at 0°C in a way that would be expected from the membrane potential. In normal incubation conditions a membrane potential of ∼−60 mv was calculated.