Premium
Neuronal Localization of Ca 2+ ‐dependent Protein Phosphorylation in Brain
Author(s) -
Sieghart Werner,
Schulman Howard,
Greengard Paul
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb11179.x
Subject(s) - phosphorylation , veratridine , kainic acid , protein phosphorylation , calcium , chemistry , biochemistry , synaptosome , incubation , microbiology and biotechnology , biophysics , biology , membrane , glutamate receptor , sodium , protein kinase a , receptor , organic chemistry , sodium channel
The cellular localization of two Ca 2+ ‐dependent protein phosphorylation systems was investigated using the kainic acid lesioning technique for the selective destruction of neurons. In one of these systems, a crude synaptosomal (P 2 ) fraction was preincubated with 32 P j for 30 min; the phosphorylation of several proteins was increased during a short subsequent incubation with veratridine plus Ca 2+ . In the second system, crude synaptosomal membranes isolated from the P 2 fraction were incubated with [γ‐ 32 P]ATP; in this system, the phosphorylation of several proteins was increased in the presence of a “calcium‐dependent regulator” plus Ca 2+ . Kainic acid lesioning greatly reduced the amount of Ca‐ + ‐dependent protein phosphorylation in both systems. The results indicate a predominantly neuronal localization for both Ca 2+ ‐dependent protein phosphorylation systems.