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Biochemical Characterization of Myelin Isolated from the Central Nervous System of Xenopus Tadpoles
Author(s) -
Trapp Bruce D.,
McIntyre Laurence J.,
Quarles Richard H.,
aka Genïchro,
Moser Ann,
Maser Hugo W.,
Webster Henry de F.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09965.x
Subject(s) - xenopus , myelin , tadpole (physics) , biology , cerebroside , biochemistry , galactolipid , galactolipids , microbiology and biotechnology , central nervous system , endocrinology , phospholipid , membrane , physics , chloroplast , particle physics , gene
Myelin isolated from the central nervous system of Xenopus tadpoles was characterized biochemically and compared with Xenopus frog and mammalian myelins. Xenopus tadpole myelin contains the characteristic protein and lipid components of mammalian myelin, although quantitative differences exist. The biochemical composition of Xenopus tadpole myelin suggests that it is an immature form of XePnopus frog myelin. Basic protein and proteolipid protein are prominent components of Xenopus myelin, but isolated tadpole myelin contains a greater proportion of higher molecular weight proteins than Xenopus frog or mature mammalian myelin. The basic protein has a higher apparent molecular weight than mammalian myelin basic protein. The levels of 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase are significantly higher in whole tadpole brain homogenate and purified myelin than in similar mammalian preparations. Tadpole myelin lipids contain a higher proportion of phospholipids and less galactolipid than mammalian myelin. Tadpole myelin galactolipids include a high (16%) percentage of monogalactosyl diglyceride, a component found in only trace quantities (0.9%) in bovine myelin.