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m‐Synephrine: Normal Occurrence in Adrenal Gland
Author(s) -
Midgley John M.,
Couch Margaret W.,
Crowley Jan R.,
Williams Clyde M.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09963.x
Subject(s) - chemistry , adrenal gland , chromatography , selected ion monitoring , phenylephrine , mass spectrometry , ion chromatography , ion , endocrinology , medicine , gas chromatography–mass spectrometry , organic chemistry , blood pressure
Gas chromatography‐mass spectrometry‐selected ion monitoring has been used to identify m‐synephrine (phenylephrine) in bovine adrenal gland. After ion exchange chromatography, m‐synephrine was converted to its N‐trifluoroacetyl‐O‐trifluoroacetoxy derivative and identified by retention time and relative intensities of the two characteristic ions at m/e 140 and m/e 455. Deuterated m‐synephrine was synthesized and used as an internal standard for quantitative analysis. Bovine adrenal gland contains 83 ng g‐ 1 (range, 33‐131) of endogenous m‐synephrine.