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The Effect of Acute Hypoxia on Synaptosomes from Rat Brain
Author(s) -
Rafajowska Urszula,
Erecifiska Maria,
Wilson David F.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09955.x
Subject(s) - hypoxia (environmental) , hypoxic hypoxia , synaptosome , mitochondrion , respiratory system , respiration , oxygen , biology , chemistry , medicine , pharmacology , endocrinology , biochemistry , central nervous system , anatomy , organic chemistry
Synaptosomes have been isolated from the brains of nonanesthetized and nembutal‐anesthetized rats subjected to 30 min hypoxia induced by breathing 7% oxygen in nitrogen. The respiratory rate was depressed in synaptosomes from starved hypoxic animals but was not significantly different from the respective control values in preparations from fed hypoxic animals, anesthetized animals, and hypoxic nonanesthetized animals allowed to recover from the hypoxic episode by 60 min of normoxic conditions. Observations are also reported concerning the levels of various metabolites in the synaptosomes isolated from the brains of the same groups of animals. It is suggested that hypoxia results in damage to the synaptosomal and/or mitochondrial membrane, which modifies substrate oxidation in the mitochondria and decreases availability of reducing equivalents for the respiratory chain. Results obtained on afflicted and recovered animals indicate that synaptosomal preparations provide a useful model for the study of hypoxic damage.