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Some Characteristics of the Night N ‐Acetyltransferase in the Rat Pineal Gland
Author(s) -
Vaněček Jiří,
Illnerová Helena
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09024.x
Subject(s) - acetyltransferase , cycloheximide , puromycin , enzyme , pineal gland , choline acetyltransferase , darkness , n acetyltransferase , chemistry , biology , endocrinology , biochemistry , acetylcholine , circadian rhythm , protein biosynthesis , botany , acetylation , gene
Following light on at night experiments, N ‐acetyltransferase level declines, at first rapidly and after 20 min more slowly. We partly characterised the night N ‐acetyltransferase and the residual N ‐acetyltransferase 20 min after light on. Both enzymes had pH optimum 6.8 and were inactivated thermally in vitro with a half time of 8 min. Pre‐treatment with cycloheximide or puromycin did not block the rise in N ‐acetyltransferase following isoprotenerol ad‐ministration shortly after light on. We propose that night N ‐acetyltransferase is one enzyme. After light on, the enzyme rapidly deactivates to a less active form. Isoprotenerol applied within a short time may partly reactivate the enzyme.