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Blood–Brain Glucose Transport in the Conscious Rat: Comparison of the Intravenous and Intracarotid Injection Methods
Author(s) -
Gjedde Albert,
Rasmussen Mette
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09012.x
Subject(s) - perfusion , anesthesia , chemistry , brain tissue , medicine
The unidirectional transfer of d ‐glucose from blood to parietal cortex tissue of the brain of awake rats was measured by single intravenous injection of tracer glucose, as well as by single intracarotid injection according to the method of Oldendorf. The maximal unidirectional blood–brain glucose transfer rate (T max ) was 407 μ mol (100 g) –1 min –1 when measured by intravenous injection, and 352 μ mol (100 g) –1 min –1 when measured by intracarotid injection. The half–saturation constants ( K m ) were 7.8 m m and 16.8 HIM, respectively. The comparison shows that the two methods give similar results when cerebral perfusion is assessed accurately.