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Effects of Denervation and Simple Disuse on Rates of Oxidation and on Activities of Four Mitochondrial Enzymes in Type I Muscle
Author(s) -
Nemeth Patti M.,
Meyer Dan,
Kark R. A. Pieter
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09009.x
Subject(s) - denervation , oxidative phosphorylation , soleus muscle , succinate dehydrogenase , medicine , biochemistry , oxidative enzyme , cytochrome c oxidase , endocrinology , mitochondrion , chemistry , enzyme , succinic dehydrogenase , biology , skeletal muscle
To differentiate the effect of muscle contractile activity from that of motor nerve on oxidative processes in type I muscle, oxidative processes were studied in muscle after immobilization and after denervation. The two processes led to similar atrophy of muscle weight and of the mean diameter of muscle fibers. Disuse of soleus muscle (type I) did not affect rates of oxidation of 14 C‐labeled substrates although these were reduced by disuse of the vastus lateralis (type II). Disuse of the soleus did not affect activities of several mitochondrial enzymes assayed by histochemical or biochemical methods. However, denervation of the soleus did lead to a fall in metabolic rates and enzyme activities. The activity of 3‐hydroxybutyrate dehydrogenase fell more than did the activities of succinic dehydrogenase, lipoamide dehydrogenase, or cytochrome–c oxidase in both homogenates and in mitochondrial fractions. These results suggest nerve may regulate mitochondrial enzymes in type I muscle. The mechanism appears to be different from that which regulates oxidative processes in type II muscle.