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Storage and Release of Noradrenaline in Hypothalamic Synaptosomes
Author(s) -
West D. P.,
Fillenz M.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09005.x
Subject(s) - synaptosome , chemistry , neuroscience , biology , biochemistry , in vitro
The noradrenaline storage capacity of vesicles in hypothalamic synaptosomes was measured by incubating them with [ 3 H]noradrenaline under saturating conditions. The normal noradrenaline content is 52% of storage capacity. Incubation or superfusion with 50 m m ‐potassium causes calcium‐dependent release from the vesicles. Such release reduces not only the vesicular content, but also the noradrenaline storage capacity. This suggests that after exocytosis vesicles cannot refill with noradrenaline.