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Hypotaurine Uptake by Brain Slices from Adult and 8‐Day‐Old Mice
Author(s) -
Oja S. S.,
Kontro P.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09002.x
Subject(s) - hypotaurine , neuroscience , neurochemistry , biology , medicine , chemistry , taurine , neurology , biochemistry , amino acid
Uptake of [ 35 S]hypotaurine by brain slices prepared from adult and 8‐day‐old mice was studied at varying temperatures, under O 2 and N 2 atmospheres, and in the presence of metabolic inhibitors and varying concentrations of hypotaurine in the incubation medium. The tissue/medium concentration gradients generated were exceptionally high for an amino acid. Hypotaurine uptake was energy‐ and temperature‐dependent, more strictly in adult mice. Uptake was saturable, containing a high‐affinity and a low‐affinity component. The estimated transport constants for the high‐affinity uptake of hypotaurine (8‐day‐old mice, 17.2 μ mol/liter; adults, 35.3 μ mol/liter) were of the same order of magnitude as the reported transport constants of putative amino acid transmitters, but the total transport capacity appears to be greatest for hypotaurine.