Premium
Nervous System‐Specific Proteins in Developing Rat Cerebral Cells in Culture
Author(s) -
Bock E.,
Yavin Z.,
Jørgensen O. S.,
Yavin E.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb09001.x
Subject(s) - glial fibrillary acidic protein , immunofluorescence , forebrain , cytoplasm , in vitro , biology , central nervous system , cell culture , astrocyte , microbiology and biotechnology , nervous system , immunohistochemistry , antibody , biochemistry , endocrinology , neuroscience , immunology , genetics
The nervous system‐specific proteins: synaptin, D1, D2, D3, glial fibrillary acidic protein (GFA) and 14‐3‐2, were quantified in dissociated cerebral cells from the foetal rat brain at various times of growth in culture. By approximately 1 week in culture, the neuronal membrane markers synaptin, D1, D2, and D3 could all be demonstrated. A maximum concentration of 10–20% for synaptin, D1, and D3 and 160% for D2, in comparison with the levels in adult forebrain, was attained during the 2nd week in vitro . The astroglial gliofilament marker GFA increased continuously, reaching by 38 days of cultivation an 18‐fold higher level than the concentration in adult forebrain. The neuronal cytoplasm marker 14‐3‐2 could be demonstrated in trace amounts, and only after more than 1 week in vitro . Neuronal cell bodies and processes stained by indirect immunofluorescence using an anti‐D2 serum were strongly fluorescent after 1 week in vitro . Immunofluorescence staining for GFA revealed a cytoplasmatic filamentous network in perinuclear areas and processes of, presumably, astroblasts.