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Influence of Synchronized Sleep on the Biosynthesis of RNA in Neuronal and Mixed Fractions Isolated from Rabbit Cerebral Cortex
Author(s) -
Giuditta Antonio,
Rutigliano Bruno,
VitaleNeugebauer Anna
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb08997.x
Subject(s) - rna , cytoplasm , cerebral cortex , cortex (anatomy) , biology , chemistry , messenger rna , endocrinology , medicine , biochemistry , anatomy , microbiology and biotechnology , biophysics , neuroscience , gene
Adult male rabbits implanted with dural electrodes were injected intraventricularly with [ 3 H]orotate and killed 1 h later. During the period of incorporation they were left undisturbed while their EEG activity was continuously monitored. In the fraction of neuronal perikarya prepared from cerebral cortex by a method developed by Satake and Abe in 1966, the relative content of radioactive RNA of the nuclear particulate showed a twofold increase in the transition from 0 to 100% synchronization. On the other hand, a slight but significant decline was observed in the corresponding cytoplasmic compartment. A marked increase in the relative content of radioactive RNA was similarly observed in the nuclear particulate prepared from the mixed cellular fraction. The corresponding cytoplasmic compartment showed a nonsignificant increase. These results indicate that during sleep neuronal nuclei accumulate newly synthesized RNA (presumably hnRNA) at a faster rate. Under the same conditions the process of RNA transfer to the cytoplasm (presumably rRNA) may be reduced. These effects may be only partly shared by other cerebral cells.