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Studies on the Wolfgram High Molecular Weight CNS Myelin Proteins: Relationship to 2′,3′‐Cyclic Nucleotide 3′‐Phosphodiesterase
Author(s) -
Sprinkle Terry Joe,
Wells Michael R.,
Garver Fred A.,
Smith Dennis B.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb07876.x
Subject(s) - myelin , myelin basic protein , biochemistry , chemistry , polyacrylamide gel electrophoresis , cyclic nucleotide phosphodiesterase , gel electrophoresis , phosphodiesterase , citrullination , enzyme , amino acid , microbiology and biotechnology , biology , arginine , neuroscience , citrulline , central nervous system
Evidence is presented that the major protein components of the high molecular weight CNS myelin proteins designated as the Wolfgram protein doublet (W1 and W2) contain the enzyme 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase (EC 3.1.4.37, CNP). CNP is a basic hydrophobic protein containing about 830 to 840 amino acid residues. When electrophoresed on SDS polyacrylamide gels, CNP appears as a protein doublet, separated by a molecular weight difference of about 2500–3000 in bovine, human, rat, guinea pig, and rabbit. A similar protein doublet has been identified as the Wolfgram proteins W2 and W1 in myelin and in the chloroform‐methanol‐insoluble pellet obtained from myelin. Moreover, the relative Coomassie blue staining intensity of the CNP2 plus CNPI protein doublet among the species examined was remarkably similar to that observed for electrophoresed myelin and chloroform‐methanol‐insoluble pellet derived from myelin. Antisera raised against purified bovine CNP recognized the W1 and W2 proteins isolated from bovine and human brain. The amino acid composition of pure bovine CNP is presented and compared with the compositions of several rat and bovine Wolfgram proteins obtained by other investigators. Our electrophoretic, compositional, and immunological data support the contention that the enzyme CNP is a major component of the Wolfgram protein doublet.