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A Radioassay for G M1 Ganglioside Concentration in Cerebrospinal Fluid
Author(s) -
Ginns Edward,
French Joseph
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb07097.x
Subject(s) - cerebrospinal fluid , ganglioside , chemistry , chromatography , medicine , biochemistry
A radioassay for the rapid determination of G M1 , ganglioside concentration in small volumes of CSF from individual patients is described. The assay utilizes the high‐affinity interaction between cholera enterotoxin and G M1 ganglioside. The lower detection limit of G M1 ganglioside by this radioassay under the described incubation conditions is 2.5 ng/ml. The radioassay‐determined lumbar CSF G M1 ganglioside concentrations in a small group of patients with diverse neurologic disorders are presented. The radioassay G M1 ganglioside concentration is in good agreement with the G M1 ganglioside concentration determined, in one patient, by the tlc‐densitometry technique.