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Effects of N ‐Ethylmaleimide Treatment on the Action Potential Na + ‐Ionophore of Cultured Neuroblastoma Cells
Author(s) -
Panini Aruna,
Blumenthal Kenneth
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb03709.x
Subject(s) - veratridine , ionophore , tetrodotoxin , sodium channel , membrane potential , biophysics , stimulation , chemistry , neuroblastoma , n ethylmaleimide , sodium , membrane , cell culture , biochemistry , endocrinology , biology , genetics , organic chemistry
: Treatment of murine neuroblastoma N18 cells with N ‐ethylmaleimide under conditions that favor diminution of membrane potential leads to a 65% increase in the rate of veratridine‐stimulated, tetrodotoxin‐sensitive Na + uptake and to a 50% decrease in the concentration of veratridine required for half‐maximal stimulation. This modification does not appear to occur in N18 cells having a normal membrane potential of approx. ‐40 mV. The data are consistent with the involvement of a sulfhydryl group in the Na + channel inactivation gate and in the conformational change in this gate which results in closing of the channel.