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ATP‐Dependent Ca 2+ Accumulation by Microvesicles Isolated from Bovine Neurohypophyses
Author(s) -
TorpPedersen Christian,
Særmark Torben,
Bundgaard Magnus,
Thorn Niels A.
Publication year - 1980
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1980.tb03690.x
Subject(s) - microvesicles , microvesicle , exocytosis , calcium , ionophore , chemistry , biochemistry , ultracentrifuge , granule (geology) , biology , membrane , paleontology , microrna , organic chemistry , gene
: Microvesicles with and without coats were isolated from bovine neurohypophyses by a series of ultracentrifugation steps. They were similar to microvesicles previously isolated from brain. In the presence of ATP and Mg 2+ , the microvesicles accumulated calcium. Oxalate stimulated and the Ca 2+ ionophore A23187 inhibited calcium accumulation. In the presence of Ca 2+ and Mg 2+ the microvesicles also demonstrated ATPase activity. As judged by SDS electrophoresis in polyacrylamide gels microvesicle protein composition was very different from that of neurosecretory granules, suggesting that they are not granule membranes “retrieved” after exocytosis. They may have an important function in regulation of Ca 24 ion concentration.