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LOCALIZATION OF GLUTAMIC ACID DECARBOXYLASE WITHIN LAMINAE OF THE RAT OLFACTORY TUBERCLE
Author(s) -
K.RIEGER NEIL R.,
HELLER JOHN S.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11732.x
Subject(s) - olfactory tubercle , glutamate decarboxylase , tubercle , lamina , anatomy , biology , rhinencephalon , chemistry , biochemistry , neuroscience , olfactory bulb , central nervous system , enzyme , genetics , bacteria , bacilli
—There are three histological layers within the rat olfactory tubercle: plexiform, pyramidal and polymorphic. We have assayed glutamic acid decarboxylase (GAD) and GABA in homogenates of frozen sections cut parallel to these layers. Consecutive sections (16 μm) were homogenized in groups and assayed for GAD or GABA. Every seventh section was stained with Toluidine Blue to monitor the depth and orientation of the plane of section. Steep variations in GAD activity (up to 6‐fold) were observed as a function of depth in the tubercle. These were paralleled by corresponding but less marked variations in GABA levels. The lowest values were found in the plexiform layer. This suggests that GAD may play a very limited role there. The highest activities were found in sections from the deepest lamina of the polymorphic layer. This is the only lamina of the tubercle that does not receive a dopaminergic input.