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ADDENDUM TO A NEW MATHEMATICAL APPROACH TO METABOLISM OF [ 14 C]GLUCOSE: THE PYRUVATE CARBOXYLASE REACTION 1
Author(s) -
Larrabee Martin G.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11714.x
Subject(s) - pyruvate carboxylase , citric acid cycle , glycolysis , biochemistry , citric acid , phosphoenolpyruvate carboxylase , metabolism , pyruvate decarboxylation , flux (metallurgy) , chemistry , enzyme , organic chemistry
—Previously published equations for analysis of [ 14 C]glucose metabolism assumed that products of glycolysis enter the citric acid cycle only through acetyl‐CoA (L arrabee , 1978). These equations are now extended to include entrance into the citric acid cycle through the pyruvate carboxy‐lase reaction as well as via acetyl‐CoA and are applied to previously reported data from dorsal root ganglia of 15‐day‐old chicken embryos. The rate of output of labelled CO 2 in the presence of [2‐ 14 C] glucose could not be accounted for if the flux rate into the citric acid cycle through the pyruvate carboxylase reaction was assumed to be more than about 10–15% of that through acetyl‐CoA. It is concluded (1) that the pyruvate carboxylase reaction is a relatively minor source of material for the citric acid cycle in these ganglia and (2) that the previous conclusions about [ 14 C]glucose metabolism, which ignored the pyruvate carboxylase reaction, need not be modified in the light of this reanalysis.