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DETERMINATION OF SOME MOLECULAR PARAMETERS OF TRYPTOPHAN HYDROXYLASE FROM RAT MIDBRAIN AND MURINE MAST CELL
Author(s) -
Kuhn Donald M.,
Rosenberg Robert C.,
Lovenberg Walter
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11700.x
Subject(s) - sedimentation coefficient , tryptophan hydroxylase , stokes radius , midbrain , tryptophan , mast cell , chemistry , ultracentrifuge , enzyme , microbiology and biotechnology , biochemistry , serotonin , biophysics , biology , endocrinology , amino acid , central nervous system , immunology , receptor , serotonergic
—The molecular parameters of tryptophan hydroxylase (EC 1.14.16.4) from rat midbrain and murine mast cell were determined by gel filtration and sucrose density gradient ultracentrifugation. The enzyme from rat midbrain has a calculated molecular weight of 220, 000, a Stokes radius of 55.6 Å, a frictional ratio of 1.28, and a sedimentation coefficient of 9.63 S. The mast cell enzyme has a calculated molecular weight of 144, 000, a Stokes radius of 50.3 Å, a frictional ratio of 1.35, and a sedimentation coefficient of 6.97 S. It appears that the tryptophan hydroxylase species from rat midbrain and murine mast cell represent distinct molecular entities.