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TWO FORMS OF ALANINE AMINOTRANSFERASE IN RAT BRAIN DURING ONTOGENY
Author(s) -
Orlick J.,
Ruščák M.,
Ruščáková D.,
Hager H.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11098.x
Subject(s) - ontogeny , cytosol , enzyme , enzyme assay , biology , mitochondrion , alanine , period (music) , biochemistry , medicine , endocrinology , amino acid , physics , acoustics
Abstract— Alanine aminotransferase activity in subcellular fractions of rat brains was investigated during ontogenic development. The activity rose from the prenatal period until adulthood, the highest increase being observed during the period of morphological metabolic and functional maturation of the brain. The rise of the total activity was due predominantly to a rise in the activity of the cytosblic enzyme; the activity of the mitochondrial enzyme did not change markedly during ontogeny. CI‐ions and elevated temperature (55°C) inhibited the activity only of the mitochondrial enzyme. Raised temperature stimulated the activity of the cytosolic enzyme while CI‐ions did not influence its activity. Our results indicate that 2 alanine aminotransferase isoenzlmes are already present in the rat brain in the prenatal period. It is assumed that the cytosolic enzyme is involved in the regulation of tissue glycol)sis and alanine formation, while the mitochondrial enzyme plays a role in the amino nitrogen transport between mitochondria and cytosol.