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THE PURIFICATION AND PROPERTIES OF PIG BRAIN CATECHOL‐ o ‐METHYLTRANSFERASE
Author(s) -
Gulliver Peter A.,
Tipton Keith F.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11094.x
Subject(s) - catechol , catechol o methyl transferase , antiserum , homogeneous , enzyme , chemistry , chromatography , affinity chromatography , glycerol , polyacrylamide gel electrophoresis , gel electrophoresis , biochemistry , biology , antibody , allele , physics , immunology , gene , thermodynamics
— The previously reported affinity chromatography technique (G ulliver & T ipton , 1978 b ) has been adapted for the large‐scale purification of pig brain catechol‐ o ‐methyltransfcrase. The enzyme prepared by this method is apparently homogeneous by polyacrylamide gel electrophoresis criteria and is stable for prolonged periods of storage at—5°C in 20% (v/v) glycerol. Kinetic measurements, molecular weight criteria and antiserum reaction suggest that the brain and liver forms of catechol‐ o ‐methyltransferase are very closely related, if not identical.