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[ 125 1] 2 α‐BUNGAROTOXIN AND [ 3 H]QUINUCLIDINYLBENZILATE BINDING IN CENTRAL NERVOUS SYSTEMS OF DIFFERENT SPECIES
Author(s) -
Salvaterra Paul M.,
Foders Renée M.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11092.x
Subject(s) - binding site , muscarinic acetylcholine receptor , biology , medicine , rana , cerebellum , endocrinology , bullfrog , cerebrum , central nervous system , microbiology and biotechnology , anatomy , biochemistry , receptor
— [ 125 I]Diiodo α‐bungarotoxin ([ 125 I] 2 BuTx) and [ 3 H]quinuclidinylbenzilate ([ 3 H]QNB) binding sites were measured in post‐nuclear membrane fractions prepared from whole brains or brain regions of several species. Species studied included Drosophila melanogaster (fruit fly), Torpedo californiea (electric ray), Carassius auratus (goldfish), Ram pipiens (grass frog), Kana cutesheiana (bullfrog), Rattus norvegicus (rat, Sprague‐Dawley), Mus muscalus (mouse, Swiss random, C58/J, LG/J), Oryctolagus cuniculus (rabbit, New Zealand Whitc), and Bos (cow). Acetyl‐CoA: choline O ‐acetyltransferase (EC 2.3.1.6) levels were also determined in the post nuclear supernatants and correlated with the number of binding sites. All species and regions except Drosophila had 16–150 fold more [ 3 H]QNB binding sites than [ 125 I] 2 BuTx binding sites. Brain regions with the highest levels of [ 125 I] 2 BuTx binding were Drosophila heads (300 fmol/mg), goldfish optic tectum (80fmol/mg), and rat and mouse hippocampus (3040 fmol/mg). The highest levels of [ 3 H]QNB binding were seen in rat and mouse caudate (1.3–1.6 pmol/mg). Lowest levels of [ 3 H]QNB and [ 125 I] 2 BuTx binding were seen in cerebellum. The utility of [ 125 I] 2 BuTx and [ 3 H]QNB binding as quantitative measures of nicotinic and muscarinic acetylcholine receptors in CNS is discussed.