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STUDIES ON AUTOIMMUNE ENCEPHALOMYELITIS IN THE GUINEA PIG—TIT. A COMPARATIVE STUDY OF TWO AUTOANTIGENS OF CENTRAL NERVOUS SYSTEM MYELIN 1
Author(s) -
Lebar René,
Vincent Christine,
Boubennec Elisabeth Fischerle
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11084.x
Subject(s) - myelin , guinea pig , trypsin , antiserum , biology , central nervous system , myelin basic protein , encephalomyelitis , antigen , microbiology and biotechnology , multiple sclerosis , immunology , biochemistry , endocrinology , enzyme
— A new CNS myelin autoantigen(s) (referred to as M2), different from the encephalitogenic basic protein (BP), can be detected with guinea‐pig demyelinating and complement fixing (CF) sera raised against guinea pig CNS tissue or myelin (L ebar et al. , 1976). M2 and BP were present in mouse, rat, rabbit, bovine and human CNS tissues when tested with guinea‐pig homologous specific antisera; they were not present in non‐CNS tissues. Both autoantigens were also detected in newborn guinea‐pig myelin and myelin‐like fractions. The CF activity of myelin with demyelinating (anti‐M2) sera was not altered by trypsin; however, absorption experiments showed that M2 was partly trypsin sensitive. Both antibodies against the trypsin sensitive and the trypsin resistant determinants of M2 were demyelinating. Both determinants of M2 were preselit in mouse, rat, rabbit, bovine‘and human CNS tissues and in guinea‐pig newborn myelin. CF BP activity of myelin was partially or even totally abolished by trypsin, but the persistent encephali‐togenicity of trypsin‐treated myelin could be attributed to non‐CF encephalitogenic peptides from BP. In accordance with recent work our results tend to support an inner localization of BP in myelin; M2, on the other hand, would be a surface antigen(s).