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ACETOACETATE AND d ‐(‐)‐BETA‐HYDROXYBUTYRATE AS PRECURSORS FOR STEROL SYNTHESIS BY CALF OLIGODENDROCYTES IN SUSPENSION CULTURE: EXTRAMITOCHONDRIAL PATHWAY FOR ACETOACETATE METABOLISM
Author(s) -
Pleasure David,
Lichtman Craig,
Eastman Sarah,
Lieb Marge,
Abramsky Oded,
Silberberg Donald
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11083.x
Subject(s) - sterol , biochemistry , labelling , enzyme , ketone bodies , biosynthesis , metabolism , biology , lyase , chemistry , cholesterol
— Oligodendroglia prepared from calf cerebral white matter were incubated with [3 ‐14 C]aceto‐acetate (AcAc), d ‐[3 ‐14 C](‐)‐beta‐hydroxybutyrate (OHbut) or 3 H 2 O. Addition to the medium of the ATP‐rate oxaloacetate lyase inhibitor (‐)‐hydroxycitrate diminished sterol labelling from OHbut and 3 H 2 O, but caused 4‐fold stimulation of sterol labelling from AcAc. The discrepancy between results with the two ketone bodies indicates the existence in oligodendroglia of an extrarnitochondrial pathway for conversion of AcAc but not OHbut to acetyl CoA. Acetoacetyl CoA synthetase, the first enzyme in this pathway, was more than 20‐fold enriched in oligodendroglia over whole brain.