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SEMIAUTOMATED FLUORIMETRIC DETERMINATION OF TYROSINE IN RAT BRAIN AND HUMAN PLASMA
Author(s) -
Evans Gwyneth,
Hartmann Margot K.,
Wood Liliana,
Gaitonde M. K.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11058.x
Subject(s) - tyrosine , trichloroacetic acid , chemistry , biochemistry , peptide , chromatography , human plasma , free fraction , plasma protein binding
— The evidence is presented to show that the fiuorimetric method of W aalkes & U denfriend (1957) cannot be used directly for the determination of free tyrosine in acid deproteinized extracts of rat brain, owing to the reaction of peptide bound tyrosine in some proteins present in the acid extracts. A method is given to obtain a fraction containing free tyrosine in the acid extracts of rat brain and to determine its tyrosine content fluorimetrically by autoanalysis. An autoanalysis method is also given for the fiuorimetric determination of free tyrosine directly in 5 0 trichloroacetic acid extracts of the plasma.