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AXOTOMY INDUCED CHANGES IN CHOLINERGIC ENZYMES IN RAT NERVE AND MUSCLES 1
Author(s) -
Ranish Norman A.,
Kiauta Tomaz,
Dettbarn WolfD.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb11042.x
Subject(s) - axotomy , acetylcholinesterase , sciatic nerve , choline acetyltransferase , anatomy , aché , chemistry , soleus muscle , endocrinology , medicine , cholinergic , free nerve ending , skeletal muscle , biology , central nervous system , enzyme , biochemistry
— The effect of axotomy on acetylcholinesterase (AChE. EC 3.1.1.7). butyrylcholinesterase (BuChE. EC 3.1.1.8) and choline acetyltransferase (CAT, EC 2.3.1.6) activities in sciatic nerve stumps above (proximal) and below (distal) the site of transection. as well as in soleus (SOL) and extensor digitorum longus (EDL) muscles, has been studied in rat. Within 2 weeks. AChE activity decreased on a per mm basis, in proximal nerve by 65%, in distal nerve by 80% and on a per whole muscle basis, in denervated muscle by 85%. BuChE activity increased in proximal nerve and distal nerve to 150% of control and decreased in muscle to 51% of control. CAT activity in the proximal nerve stump was reduced to 70% of control and in the distal stump to less than 1% within 1 week. CAT activity in muscle decreased to 20 and 30% of control for soleus and EDL respectively during a 14 day period. The reduction in AChE and CAT activity observed in the proximal nerve segment may reflect changes in the synthesis, as well as the transport and local degradation of these enzymes. Previous studies on axotomy induced changes have not examined the simultaneous changes in proximal and distal nerve as well as denervated muscle in a single animal species.