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RELATIONSHIP BETWEEN THE 4‐AMINOBUTYRATE BYPATH AND THE OXIDATION OF 2‐OXOGLUTARATE IN RAT BRAIN MITOCHONDRIA
Author(s) -
LopesCardozo M.,
Albers R. W.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb05272.x
Subject(s) - mitochondrion , biochemistry , chemistry , citric acid cycle , biology , metabolism
— A method is described to evaluate simultaneously the contributions of 2‐oxoglutarate oxidation and the GABA bypath to succinate production in isolated rat brain mitochondria. 2‐Oxoglutarate oxidation is under respiratory control whereas the activity of the GABA shunt is but slightly affected by the mitochondrial energy state. The oxidation of GABA is half‐maximal with 5m m ‐GABA. GABA does not affect 2‐oxoglutarate oxidation. 1 m m ‐2‐oxoglutarate is optimal for GABA oxidation, whereas higher concentrations inhibit the shunt activity. The rate of GABA oxidation observed in vitro (5 nmol/min.mg mitochondrial protein) is comparable to the activity of the shunt under in viuo conditions. The control and the compartmentation of GABA oxidation are discussed.